我院近年来主要科研成果

发布者:王维发布时间:2018-05-17浏览次数:2453

(一)科技奖励

  1. 鄂豇豆二号早熟优质丰产途径及机理研究,湖北省科技进步三等奖,2008

  2. 武汉优势农作物品种分子图谱识别技术与应用,武汉市科技进步二等奖,2010

  3. 豇豆新品种选育,武汉市职工技术创新成果一等奖,2010

  4. 豇豆新品种选育,湖北省科技进步二等奖,2011

  5. 豇豆新品种选育,武汉市科技进步二等奖,2011

  6. 菊花耐热种质创新与应用,武汉市科技进步三等奖,2012

  7. 地方鹅与朗德鹅杂交提高肥肝鹅繁殖性能的研究,湖北省科技进步三等奖,2012

  8. 地方鹅与朗德鹅杂交提高肥肝鹅繁殖性能的研究,武汉市科技进步三等奖,2012

  9. 豇豆新品种及配套技术产业化,“十一五” 湖北高校科技成果转化提名奖,2012

  10. 大别山区夏枯草规范化种植及产业链构建,湖北省科技进步三等奖,2015

  11. 夏枯草优质野生种质资源的筛选及推广应用,湖北省科技进步三等奖,2017

      

    (二)已获得的发明专利与新品种

  12. 基于基因组RAPD分析的豇豆品种分子鉴定方法(ZL200510018593.5

  13. 基于盐溶蛋白图谱技术的豇豆品种分析鉴定方法(ZL200510018594.X

  14. 冷季型草坪草肥料(ZL200510018362.4

  15. 暖季型草坪草肥料(ZL200510018361.X

  16. 豇豆的化学调控栽培方法(ZL200510018546.0

  17. 辣椒的化学调控栽培方法(ZL200510018547.5

    高效优质猪肉生产符合预混料(ZL 200610019550.3

    无公害茶用菊花栽培方法(ZL200610018988.X

    茶用菊花专用肥料(ZL200610018986.0

    茶用菊花无激素组织培养获得脱毒(ZL200610018987.5

  18. 豇豆基因型耐盐能力的技术鉴定方法(ZL200810048423.5

  19. 基于ISSR分子标记构建中国长豇豆遗传资源数据库的方法及其应用(ZL200810197366

  20. 豇豆基因型耐湿能力的技术鉴定方法(ZL200810048424X

  21. 茶用菊花专用肥料(ZL200610018986.0

  22. 乌塌菜有机生态型无土栽培方法(ZL201110154691.7

    一种功能性啤酒及其酿造方法(ZL201110183253

    一种牙基质海绵剂及其制备方法(ZL201110129457.9

    一种微生物基因克隆的方法(ZL201110216425.2

    一种功能性啤酒及其酿造方法(ZL201110183253

    种红小豆生态种植方法(ZL201210153938

    一种大型底栖动物筛选网具(ZL201220497729.0

    一种抽滤装置(ZL201220376959.1

    一种用蓝藻制作有机肥的方法以及发酵反应器(ZL201210417690

    CODCr批量消解装置(ZL201210003202

    一种用蓝藻制作有机肥的方法以及发酵反应器(ZL201210417690

  23. 濒危蕨类植物水蕨的繁育方法(ZL201210075022

  24. 利用大肠杆菌表达系统制备重组人神经生长因子的方法(ZL201210278039.0

    莼菜的迁地保护栽培方法(ZL 2012 1 0050530.1

    一种预防EV71基因工程蛋白疫苗及制备方法(ZL201310066245.X

  25. 一种长薄鳅四碱基重复单元微卫星分子标记的构建方法及其应用(ZL 201310070294.0

    一种菜豆植物凝集素凝血活性定量检测方法(ZL201310532286

  26. 水稻的白叶枯病抗性的鉴定方法(ZL201310091652.6

  27. 水稻的白叶枯病抗性的鉴定方法.ZL201310093287.2

  28. 豇豆叶绿体微卫星分子标记的多态性引物及其筛选方法、鉴定亲缘关系的方法.

    ZL 201410074030.7

    菠菜有机生态型无土栽培方法(ZL201410374872.4)

    一种夏枯草的杂交栽培方法ZL 2014 1 0424496.5

  29. 利用miRNA162b基因早期精确预报水稻白叶枯病的方法. ZL201410307328.8

  30. 利用miRNA827基因预报水稻白叶枯病的方法.ZL201410309695.1

  31. 一种利用miRNA1430基因预报水稻白叶枯病的方法(ZL201410309693.2

  32. 利用miRNA167c基因早期精确预报水稻白叶枯病的方法(ZL201410307326.9

  33. 一种快速、准确的检测杂交水稻种子纯度的方法(ZL201210280185.3

  34. 鄂豇豆2, 鄂种审证字第176

  35. 鄂豇豆6, 鄂审菜2006002

  36. 鄂豇豆7, 鄂审菜2007004

  37. 鄂豇豆12, 鄂审菜2013002

  38. 鄂菜豆1号,鄂审菜2016009

  39. 鄂豇豆14,鄂审菜2016008

  40. 水稻新品种科优73 CNA20100473.2

  41. 水稻新品种R7723. CNA20100474.1

    (三)代表性科研论文

尚蕊, 吴华, 郭瑞. 不同菜豆品种4种抗营养因子水平差异分析. 园艺学报, 2015, 42(1): 2163-2173.

李佳楠, 杨薇, 彭娜等. 菜豆毒性分析及毒性预测模型建立. 中国农业科学, 2015, 48 (4): 727-734.

熊飞, 刘红艳, 段辛斌等. 长江上游朱杨溪江段圆筒吻鮈种群参数和资源量. 生态学报, 2015, 35(22): 7320-7327.

Chen C Y et al. Genetic Analysis of Salt Stress Responses in Asparagus Bean (Vigna unguiculata (L.) ssp. sesquipedalis Verdc.). Journal of Heredity, 2007.

Chen C Y et al.Physiological and biochemical responses to salt stress of seedlings of Vigna unguiculata (L.) ssp. sesquipedalis Verdc. from tolerant and sensitive genotypes. International Society for Horticultural Sciences, 2008 (ISTP).

Chen C Y et al. Differentiation and phylogenetic relationship among different cultibars of asparagus bean (Vigna unguiculata (L.) ssp. sesquipedalis) assessed using ISSR markers.Nordic Journal of Botany, 2010, 28: 251-256.

Zeng C L et al. High-Throughput Discovery of Chloroplast and Mitochondrial DNA Polymorphisms in Brassicaceae Species by ORG-EcoTILLING.. PLOS ONE 2012, 7(11): e47284.

Zeng C L et al. Physiological effects of exogenous nitric oxide on potherb mustard (Brassica juncea, Coss.) seedlings under NaCl stress. Biologia Plantarum, 2011, 55: 345-348.

Zeng C L et al. The Physiological Responses of Carnation Cut Flowers to Exogenous Nitric Oxide. Scientia Horticulturae, 2011, 127: 424-430.

Zeng C L et al. Physiological Effects of Exogenous Nitric Oxide on Brassicachinensis seedlings under

NaCl stress. RSETE, 2011 (EI).

Peng H et al. Rice Xa21 primed genes and pathways that are critical for combating bacterial blight infection. Scientific Reports, 2015

Peng H, Zhang J. Commercial high-throughput sequencing and its applications in DNA analysis

Biologia, 2009, 64(1): 20-26.

Peng, H et al. MicroRNA profiles and their control of male gametophyte development in rice. Plant Mol. Biol., 2012, 1-18.

Jiang X W et al. High Semi-Lethal Temperature and Heat Tolerance of Tea Chrysanthemum. Acta Hort. , 2013, 977: 369-376

Jiang X W et al. Multiple events of allopolyploidy in the evolution of the racemose lineages in Prunus (Rosaceae), based on integrated evidence from nuclear At103 and ITS and plastid data. PLOS ONE,2016, 11(6): e0157123

FengT. et al. Effects of Pyrethroids and Endosulfan on Fluidity of Mitochondria Membrane in Chilo suppressalis (Walker). Pesticide Biochemistry and Physiology, 2009, 95:72-76.

Dai X G. et al. Improvement of isolated microspore culture of ornamental kale (Brassica oleracea var. acephala): effects of sucrose concentration, medium replacement, and cold pre-treatment. Journal of Horticultural Science and Biotechnology, 2009, 84: 519-525.

Dai X G et al. High frequency plant regeneration from cotyledon and hypocotyl explants of ornamental kale (Brassica oleracea var. acephala). Biologia Plantarum, 2009, 53: 769-773.

Hu J et al. Identification of conserved microRNAs and their targets in the model legume Lotus japonicus. Journal of Biotechnology,2013, 164: 520-524.

Hu J et al. Characterization of conserved microRNAs from five different cucurbit species using computational and experimental analysis. Biochimie,2014, http: // dx.doi.org/ 10.1016/j.biochi. 2014.03.002.

Pan L et al. Development of 12 chloroplast microsatellite markers in Vigna unguiculata (Fabaceae) and amplification in Phaseolus vulgaris. Applications in Plant Sciences, 2014, 2 (3): 1300075.

Dong Y Het al. Genetic structure and gene flow in the endangered aquatic economic crop Brasenia schreberi J. F. Gmel. (Nymphaeaceae) in China.PIAGENG, 2013(EI).

Dong Y Het al. Effect of habitat modification on the distribution of the endangered aquatic fern Ceratopteris pteridoides (Parkeriaceae) in China, and conservation strategy. American Fern Journal. 2012, 102(2):136-146.

Dong Y Het al. Genetic variation and gene flow in the endangered aquatic fern Ceratopteris pteridoides in China, and conservation implications.Annals Botanici Fennici.2010, 47(1): 34-44.

Dong Y Het al. An investigation of genetic variation in the endangered aquatic fern Ceratopteris thalictroides (Parkeriaceae) in China : implications from RAPD and ISSR data.Botanical Journal of the Linnean Society, 2008,157: 657-671.

Jiang XW et al. Studies on Heat Tolerance of Ground-Cover Chrysanthemum. Acta Horticulturae (ISTP), 2008, 766: 89-96.

Li J N et al. Demineralized Dentin Matrix Composite Collagen Material for Bone Tissue Regeneration. Journal of Biomaterials Science, Polymer Edition, 2013, 24(13):1519-1528.

Li J N et al. Viral specific cytotoxic T cells inhibit the growth of TfR-expressing tumor cells with antibody targeted viral peptide/HLA-A2 complex. Cellular Immunology, 2010, 263(2): 154-160.

Xiong F et al. Accumulation and depuration of Copper and Zinc in the Freshwater Mussel Cristaria Plicata (Leach) under Laboratory Conditions.Proceedings 2011 International Conference on Computer Distributed Control and Intelligent Environmental Monitoring, 2011(EI).

Xiong F et al. Threatened fishes of the world: Gymnocypris przewalskii (Kessler, 1876) (Cyprinidae: Schizothoracinae). Environmental biology of fishes, 2010, 87(4): 351-352.

Xiong F et al. Length–weight and length–length relationships of 20 fish species from the upper Yangtze River, southwest China. Journal of Applied Ichthyology, 2017, 33(4): 835-838.

Liu H Y et al. Isolation and characterization of polymorphic microsatellite loci from elongate loach (Leptobotia elongate), a threatened fish species endemic to the Yangtze River. Conservation Genetics Resources, 2012, 4: 129-131.

Liu H Y et al. A first set of polymorphic microsatellite loci isolated from Rhinogobio cylindricus. Conservation Genetics Resources, 2012, 4: 307-310.

Liu H Y et al. Isolation and characterization of 19 polymorphic microsatellite loci from Neosalanx taihuensis, a rapidly invasive and adaptative species. Biochemical Systematics and Ecology, 2015, 61:121-123.

Liu H Y et al. Low population differentiation revealed in the highly threatened elongate loach (Leptobotia elongata, Bleeker), a species endemic to the fragmented upper reaches of the Yangtze River. Biochemical Systematics and Ecology, 2017,70: 22-28

Han X et al. Expression and purification of enterovirus type 71 polyprotein P1 using pichia pastoris system. Virologica Sinica, 2012, 27(4): 254-258.

Han X et al. Characteristic of the enterovirus 71 VP1 protein as vaccine candidate. Journal of Medical Virology, 2014 .

Han X et al. Characterization of the enterovirus 71 P1 polyprotein expressed in Pichia pastoris as a candidate vaccine. Human Vaccines & Immunotherapeutics , 2014, 10(8).

Pan L et al. A high-resolution genetic map derived from RAD sequencing and its application in QTL analysis of yield-related traits in Vigna unguiculata. Frontiers in Plant Sciences, 2017 (online).

Pan L et al. Bioinformatic identification and expression analysis of Nelumbo nucifera MicroRNA and their targets. Applications in Plant Sciences, 2015, 3(9):1500046.

Pan L et al. Development of 12 chloroplast microsatellite markers in Vigna unguiculata (Fabaceae) and amplification in Phaseolus vulgaris.Applications in Plant Sciences, 2014, 2(3): 1300075.

Rui Shang et al. The diversity of four anti-nutritional factors in common bean. Horticultural Plant Journal, 2016: 97-104.

Ning T T et al. Gut Microbiota Analysis in Rats with Methamphetamine-Induced Conditioned Place Preference. Front Microbiol, 2017, 8: 1620.

Ning T T et al. Engineering Microbial Fatty Acid Biosynthesis Pathways to Make Anvanced Biofuels, Biofuels, from mircobes to moleculars, Xuefeng Lu, Caister Academic Press, 2014.

Xia J et al. An accurate and efficient method for large-scale SSR genotyping and applications, Nucleic Acids Res, 2017, doi: 10.1093/nar/gkx093.

Yuan Xu, Siyi Pan. Effects of various factors of ultrasonic treatment on the extraction yield of all-trans-lycopene from red grapefruit (Citrus paradise Macf.). Ultrasonics Sonochemistry, 2013, 20(4): 1026-1032.

Zhou L H et al. Developmental changes for the hemolymph metabolome of silkworm (Bombyx mori L.) Lihong Zhou; Huihui Li; Huiru Tang. Journal of Proteome Research, 2015, 14 (5): 2331–2347.

Sun X X et al. In Vivo Targeting and Imaging of Atherosclerosis Using Multifunctional Virus-Like Particles of Simian Virus 40. Nano Letter 2016,16: 6164-6171.

Wang H B et al. Rapid and environmentally-friendly extraction of carotenoids from Blakeslea trispora. Biotechnology letters, 2015, 37(11): 2173-2178.

Wang H B et al. Improved β-carotene and lycopene production by Blakeslea trispora with ultrasonic treatment in submerged fermentation. Zeitschrift für Naturforschung C. 2014, 69c: 237- 244.

Wang H B et al. High-quality lycopene overaccumulation via inhibition of γ-carotene and ergosterol biosyntheses in Blakeslea trispora. Journal of Functional Foods, 2014,7: 435-442.

Wang H B et al. Oxidative stress response of Blakeslea trispora induced by H2O2 during β-carotene biosynthesis. Journal of industrial microbiology & biotechnology, 2013, 41: 555-561.

Liu Q et al. Characterization of a novel mosquitocidal strain of Bacillus thuringiensis serovar aizawai which harbors a rolling-circle replication plasmid, pBt1-3. Journal of Asia-Pacific Entomology, 2013, 16(3): 257-261.

Liu Q et al. Construction and characterization of an antifungal recombinant Bacillus thuringiensis with an expanded host spectrum. Journal of Microbiology, 2012, 50(5): 874-877.

  

(四)代表性结果

柳翠鄂豇豆7号(矮虎)早翠

鄂豇豆6号(柳翠)鄂豇豆7号(矮虎)鄂豇豆2号(早翠)

  

中英文

  

不同菜豆品种4种抗营养因子水平差异分析(园艺学报,2015

Fig 1

基于质体DNA数据的李属系统发育关系Fig 4

李属进化中的异源多倍体事件PLOS ONE, 2016

figure 1figure 7

ORG-Ecotilling技术检测芸薹属作物叶绿体和线粒体基因组多态性(PLOS ONE, 2012

病毒肽/HLA-A2CD71单链抗体融合基因及融合蛋白结构图(Cellular Immunology2010

fig4fig5

脱钙人牙基质复合材料植入后组织切片染色图(J BIOMAT SCI-POLYM E, 2013

  

GATGAGTCCTGAGTAATGACTGAGTGGGATATTGTTACTGACACATATTGGGGCTGTGTTATAAGGGCTGCCAGGCCCACCTGTCACATAGGAATATGAAGCGAGGGTAAAAGAGGAGAGGGGAGACGATGGTAATAGTAAGAGGGAGGAAAAACTATACAGAGAGAAAGAGAGAAAGTAAATGAGCCTCTCTCCTGAGATTGACCCTCCTCTCGTCTCTCGCCTGTCTGTCAGTGCTGCAGGTGTCTGTGCGGTCAGTGAGAACGAGTGTGTGCTCCACTGGAGCATGCAGCTAGACCAGGAAGTGTTCTGAATACTGAAAACACAAACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACACTCAAATACACAGGAAACACTGAGAGGAGAGCAGATATAAAAAAAAAGCTGACCTGCTCTGAATGCTTACTCAGGACTCAT

圆筒吻鮈的一条完美型重复微卫星序列图示(Conservation Genetics Resources2012a)

  

不同位点的微卫星引物扩增长薄鳅DNA的银染图(Conservation Genetics Resources2012b

  

  

濒危鱼类青海湖裸鲤图(Environmental biology of fishes, 2010)

NO对香石竹切花保鲜的影响(Scientia Horticulturae, 2011

VP1蛋白的表达与纯化(Journal of Medical Virology, 2014

濒危植物粗梗水蕨5个自然种群的UPGMA类图(Annals Botanici Fennici2010

  

tu-2副本

羽衣甘蓝游离小孢子培养技术研究(Journal of Horticultural Science & Biotechnology2009

豇豆叶绿体基因组

几个豇豆品种的叶绿体cpSSR分子标记指纹图谱(Applications in Plant Sciences, 2014

豇豆豆荚产量性状的QTL定位

豇豆亲本及杂交后代F23的豆荚产量性状变异

豇豆遗传图谱与菜豆基金组比较的Circos豇豆遗传图谱与红豆基因组的Circos图(Frontiers in Plant Sciences, 2017

濒危鱼类青海湖裸鲤图(Environmental biology of fishes, 2010)

长江上游长薄鳅群体结构STRUCTURE分析图(Biochemical Systematics and Ecology2017